Sambucus nigra (SNA I) (Agarose)

Sambucus Nigra Lectin is a 150,000 molecular weight tetrameric glycoprotein consisting of four disulfide-linked chains, two of about 36,000D and two of about 38,000D. Sambucus nigra lectin binds preferentially to sialic acid attached to terminal galactose in (a-2,6), and to a lesser degree, (a-2,3), linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalacto-samine. SNA has been reported to inhibit cell-free protein synthesis. Agarose bound Sambucus Nigra Lectin is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10e7 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose provides a very hydrophilic spacer arm between the protein and the matrix. This ensures maximum expression of the carbohydrate binding activity of the lectin. The linkage is very stable over a range of pH values and, unlike cyanogen bromide linkages, proteins are not leached off the gel by Tris or other routinely used buffers. In addition, residual charges generated during cyanogen bromide conjugation which can produce nonspecific binding are not present on the gel following our coupling procedure.

Suitable for use in ELISA. Other applications not tested.

Optimal dilutions to be determined by the researcher.

≥1.5mg fetuin/ml gel

500mM lactose in buffered saline followed by 500mM lactose in acetic acid 3mg lectin/ml gel

2x10e7

May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Sambucus nigra (elderberry) bark

~3mg/ml

Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.1mM Ca++, 0.08% sodium azide, 20mM lactose. Labeled with Agarose.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.