PureStain Mouse-on-Mouse Kits for IHC
Detect Mouse Antibody on Mouse Tissue
- Polymer based conjugation, biotin free
- Improved formula for best sensitivity and clean background
- Total blocking time : 35 min
- Tested on mouse and rat tissue
Antigen detection with primary antibody of the same species as the test tissue yields high background when indirect detection method is used. This severely limits the use of murine monoclonal antibody on mouse tissues.
The PureStain Mouse-on-Mouse HRP and AP kits are composed of a polymer detection system designed for screening antigens in mouse tissue sections using mouse primary antibodies with no background.
The PureStain Mouse-on-Mouse kits contain a two-step block that reacting with endogenous mouse IgG in mouse tissue.
The PureStain Mouse-on-Mouse kits are biotin-free which eliminates the need for blocking endogenous biotin.
The PureStain Mouse-on-Mouse kits are designed for users to choose from several available chromogens. We also sell the PureStain Mouse-on-Mouse Kit Blocking A & B solutions if you want to do immunofluorescence staining or use your own antibodies.
Mouse anti-nucleophosmin/B23 was used to stain paraffin embedded mouse kidney using PureStain Mouse-on-Mouse kit.
Picture on the left shows no staining with negative control antibody. Picture on the right shows strong positive nuclear stain when stained with mouse anti-nucleophosmin
PureStain MOM Kits
Polymer HRP or AP
HRP for AEC