Custom service : Antibody validation in IHC on TMA

Custom service : Antibody validation in IHC on TMA

This analysis is realized in normal and cancerous formalin-fixed and paraffin-embedded (FFPE) Tissue Micro-Arrays (TMA).

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This is interesting to :
- characterize a target expression in tissues from healthy and tumor-affected donors
- find a correlation between tumor phenotype and pronostic

Standard protocol :
- Developing an immunohistochemistry protocol specific to your antibody
- Staining of healthy tissues and multi-tumoral tissues on TMA slides
- Analysis of results
- Option 1 : Staining of tissue specific TMA (breast, lung, colon, prostate, pancreas, bladder, kidney, ovary, endometer, head and neck)
- Option 2 : Staining of TMA containing primary and metastatic tumors.

Key numbers :
- Number of available tumors : > 100
- Numbre of tissues/patients per TMA slides from healthy donors : 76/8
- Numbre of tissues/patients per TMA slides of tumoral tissues : 120/50-60

Related services available :
=> Peptide synthesis/Recombinant production
=> Polyclonals production
=> Monoclonals production
=> Antibody labeling
=> Antibody purification
=> Antibody fragmentation
=> Antibody refolding