Maackia amurensis Lectin II (MAL II)

Cat# M1061-15-2mg

Size : 2mg

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Grade
Molecular Biology Grade

Shipping Temp
Blue Ice

Storage Temp
4°C

Amur Maackia
Maackia amurensis lectin II (MAH) is the hemagglutinin isolectin from Maakia seeds. It is a glycoprotein consisting of four subunits. The molecular weight of the lectin is about 140,000 resolvable by SDS gel electrophoresis into two bands of 34,000 and 36,000D. The isoelectric point of this lectin is about pH 4.7 but demonstrates some charge heterogeneity.

Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike SNA which seems to prefer structures with (a-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (a-2,3) linkage. While fetuin is a poor inhibitor, glycophorin is a very potent inhibitor of MAL II binding. Tissue staining patterns are also very different among MAL I, SNA and MAL II.

Applications:
Suitable for use in Glycobiology and Mitogenic Stimulation. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Sugar Specificity:
Sialic Acid

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Source
Maackia amurensis seeds

Purity
Homogeneous by SDS-PAGE

Concentration
~2mg/ml

Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM Ca2+.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.