Erythrina cristagalli (ECA) (Biotin)
Cat# E3453-20D-1mg
Size : 1mg
Brand : US Biological
Conjugate
BiotinGrade
PurifiedShipping Temp
Blue IceStorage Temp
-20°CCockspur Coral Tree
Pure Erythrina cristagalli lectin (ECA) from coral tree. Biotin conjugated.
Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.
Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
Carbohydrate Specificity: Gal beta(1,4)GlcNAc
Inhibitory Carbohydrate: N-acetyllactosamine > lactose > N-acetylgalactosamine > galactose
Activity: 1-2ug/ml will agglutinate neuraminidase treated human erythrocytes.
Chemical Used for Conjugation: Biotinyl N-hydroxysuccinimide ester (BNOHSE).
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified
Concentration
~1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder in 0.01M phosphate, 0.15M sodium chloride, pH 7.2-7.4. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml. Labeled with Biotin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.