TNF alpha ELISpot Assay Kit (Rat)

Cat# OKDB00039-1x96wells

Size : 1x96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva's Rat TNF alpha ELISpot Kit is based on standard sandwich ELISA methodology. A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.
ELISA Kit ComponentReagents sufficient for 96 Wells:
1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells)
2. Biotinylated detection antibody
3. Streptavidin-Alkaline Phosphatase conjugate
4. Bovine Serum Albumin
5. Ready to use BCIP/NBT substrate buffer
Reconstitution and Storage2 to 8C, Do not use past expiration date.
Sample TypeCell Suspensions
Assay InfoAssay Methodology: Quantitative Sandwich ELISpot Immunoassay
Gene SymbolTNF
Gene Full Nametumor necrosis factor
Alias SymbolsTnfa, RATTNF, TNF-alpha
NCBI Gene Id24835
Protein Nametumor necrosis factor
Description of Targetacts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]
Uniprot IDP16599
Protein Accession #NP_036807.1
Nucleotide Accession #NM_012675.3
Protein Size (# AA)235
Molecular Weight26 kDa