IFN gamma ELISpot Assay Kit (Rat)
Cat# OKDB00037-5X96wells
Size : 5x96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Rat |
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| ELISA Kit Duration | < 4 Hours |
| ELISA Kit Principle | Aviva's Rat IFN gamma ELISpot Kit is based on standard sandwich ELISA methodology. A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope. |
| ELISA Kit Component | Reagents sufficient for 96 Wells: 1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells) 2. Biotinylated detection antibody 3. Streptavidin-Alkaline Phosphatase conjugate 4. Bovine Serum Albumin 5. Ready to use BCIP/NBT substrate buffer |
| Reconstitution and Storage | 2 to 8C, Do not use past expiration date. |
| Sample Type | Cell Suspensions |
| Assay Info | Assay Methodology: Quantitative Sandwich ELISpot Immunoassay |
| Gene Symbol | IFN |
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| Gene Full Name | interferon gamma |
| Alias Symbols | If2f, IFNG2 |
| NCBI Gene Id | 25712 |
| Protein Name | interferon gamma |
| Description of Target | an immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006] |
| Uniprot ID | P01581 |
| Protein Accession # | NP_620235.1 |
| Nucleotide Accession # | NM_138880.2 |
| Protein Size (# AA) | 156 |
| Molecular Weight | 18 kDa |