Change your vision of PCR, move to digital
Optimized master mix for microfluidic chip digital PCR
N-Flux 5X Digital PCR Master Mix is a high-performance, universal probe master mix specially formulated for microfluidic chip-based digital PCR (cdPCR) platforms for maximum end-point fluorescence and superior separation between positive and negative partitions.
- Sensitive, robust, and reproducible performance in dPCR and qPCR assays
- Minimizes interactions with hydrophobic plastic surfaces for optimal sample partitioning
- Maximizes end-point fluorescence for superior separation between positive and negative partitions
- Validated for multiplex or singleplex, genotyping, and copy number variation dPCR and qPCR
- Suitable for all fluorescent probe types, including TaqMan®, BHQ®, and molecular beacons
- 5X concentration facilitates PCR with dilute or large sample volumes
- Formulated with Cheetah HotStart Taq for fast cycling protocols
A Novel Master Mix Optimized for Microfluidics
N-Flux 5X Digital PCR Master Mix is designed to optimize partitioning and minimize sample adsorption during flow through the microfluidic channels of the chips and plates used in cdPCR. The master mix maximizes end-point fluorescence for superior separation between positive and negative partitions resulting in sensitive and accurate results. N-Flux 5X Digital PCR Master Mix has been validated in singleplex and multiplex probe-dPCR reactions. Using the Combinati Absolute Q digital PCR platform, N-Flux 5X Digital PCR Master Mix reproducibly performed genotyping and copy number variation analysis with quantitative discrimination. The master mix is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like molecular beacons). Contact us to inquire about custom formulations for your platform development project.
High-resolution, multiplex copy number variation analysis performed using N-Flux 5X Digital PCR Master Mix on the COMBiNATi Microfluidic Array Partitioning (MAP16) device. Superior partitioning results in quantitative determination of the copy number of a low abundance SNP variant and a high abundance control on human gDNA, with excellent separation between positive and negative wells.
While N-Flux 5X Digital PCR Master Mix is formulated to address the complexities of flow through microfluidic chambers, the 5X concentration can also simplify the work flow for qPCR users. The high concentration gives flexibility and facilitates PCR analysis of large sample volumes or samples that are too dilute for traditional 2X master mixes. See our Forget-Me-Not Master Mixes for EvaGreen® dye-based or probe-based quantitative real-time PCR.
The kit contains master mix with Cheetah HotStart Taq DNA Polymerase and dNTPs in a specially formulated buffer and includes a separate vial of ROX passive reference dye for optional use.
Cheetah HotStart Taq DNA Polymerase
Biotium's proprietary chemically modified hot-start DNA Polymerase. Cheetah Taq is completely inactive at room temperature, but is fully activated in 2 minutes at 95°C with high activity recovery, making it particularly suitable for fast PCR.