ELISPOT kits

ELISPOT kits


Enzyme-linked immunospot (ELISpot) assays are highly sensitive immunoassays that measure the frequency of individual cytokine-secreting cells. In these assays, cells are cultured on a surface coated with a specific capture antibody in the presence or absence of stimuli. Proteins, such as cytokines, that are secreted by the cells will be captured by the specific antibodies on the surface. After an appropriate incubation time, cells are removed and the secreted molecule is detected using a detection antibody in a similar procedure to that employed by the ELISA. The detection antibody is either biotinylated and followed by a streptavidin-enzyme conjugate or the antibody is directly conjugated to an enzyme. By using a substrate with a precipitating rather than a soluble product, the end result is visible spots on the surface. Each spot corresponds to an individual cytokine-secreting cell.

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RUOCE / IVD
  • Unconjugated 14777
  • Biotin 113
  • AP 38
  • HRP 37
  • Low Endotoxin/Azide Free 24
  • FITC 9
  • APC 6
  • PE 4
  • RPE 2
  • TRITC 2
  • AbFluor™ 488 1
  • BGAL 1
  • human 7378
  • mouse 4043
  • rat 2625
  • all species 378
  • cow 357
  • pig 295
  • dog 172
  • rabbit 160
  • chicken 112
  • monkey 63
  • horse 62
  • sheep 41
  • guinea pig 33
  • goat 19
  • cat 3
  • hamster 1
  • human 838
  • mouse 227
  • rat 152
  • goat 134
  • monkey 28
  • rabbit 19
  • horse 8
  • cow 4
  • chicken 2
  • hamster 2
  • sheep 2
  • Primary antibody 14819
  • ELISA/ELISPOT 1081
  • Secondary antibody 188
  • Discontinued 7
  • kit 4
  • Protein/peptide 2
  • Buffers and reagents 1
  • Equipment 1
  • ELISA 15871
  • ELISPOT 15015
  • Immunoassay 14546
  • IHC 278
  • WB 244
  • FC 187
  • ICC 177
  • IP 54
  • ISH 31
  • FA 24
  • FLISA 23
  • Stimulation 20
  • Neutralization 17
  • Cell depletion 8
  • Multiplex assay 8
  • Purification 7
  • Chemiluminescence 6
  • Detection 6
  • Microscopy 4
  • IF 3
  • Assay 2
  • Blocking 2
  • Proximity Ligation Assay 1
  • Quality Tested:
    ELISA
    FLISA

    Report in literature:
    ELISpot
    FC
    ICC
    WB
    Multiplex
    1
  • Polyclonal 168
  • Monoclonal 23
  • 23G3 7
  • 1B4B1 6
  • HP6029 5
  • [B-B1] 5
  • [B-B51] 5
  • [B-C49] 5
  • [B-C7] 5
  • [B-E48] 5
  • [B-E8] 5
  • [B-F60] 5
  • [B-F7] 5
  • [B-G1] 5
  • [B-G46] 5
  • [B-P24] 5
  • [B-P6] 5
  • [B-R14] 5
  • [B-T10] 5
  • 2A8F4 4
  • G11C5 4
  • H2 4
  • JES6-5H4 4
  • TRFK5 4
  • XMG1.2 4
  • 11B11 3
  • 1A7 3
  • 1D11.16.8 3
  • 3E6 3
  • 48110 3
  • C3F12 3
  • CC301 3
  • CC318 3
  • JES5-16E3 3
  • MD-1 3
  • MP4-25D2 3
  • MP6-XT3 3
  • [31-7-4] 3
  • [B-C48] 3
  • [B-G5] 3
  • 5D10 2
  • ALF-161 2
  • B27 2
  • BVD2-21C11 2
  • BVD2-23B6 2
  • BVD4-1D11 2
  • BVD6-24G2 2
  • CC330 2
  • DB-12 2
  • JES1-39D10 2
  • JES1-5A10 2
  • JES10-5A2 2
  • JES3-12G8 2
  • JES3-9D7 2
  • JES5-2A5 2
  • JES6-1A12 2
  • MAb1 2
  • MAb11 2
  • MP1-22E9 2
  • MP2-43D11 2
  • MP2-8F8 2
  • MP5-20F3 2
  • MP5-32C11 2
  • MP6-XT22 2
  • MQ2-13A5 2
  • MQ2-39C3 2
  • QS-5 2
  • TC11-18H10 2
  • TC11-8H4 2
  • TRFK4 2
  • [5D10] 2
  • [B-B13] 2
  • [B-D48] 2
  • [B-E4] 2
  • [B-G28] 2
  • [B-S10] 2
  • [B-S23] 2
  • [B-T21] 2
  • [B-T24] 2
  • [B27] 2
  • [CC301] 2
  • [CC318] 2
  • [CC330] 2
  • [HP6002] 2
  • [HP6029] 2
  • [JES1-39D10] 2
  • [MP6-XT22] 2
  • 31-7-4 1
  • 4F12 1
  • 5B5 1
  • A5D5 1
  • B-B13 1
  • B-F7 1
  • B-G5 1
  • B-P24 1
  • B-S10 1
  • DB-1 1
  • DB-10 1
  • DB-14 1
  • DL-8 1
Cat#
Description
Cond.
Price Bef. VAT
KA3937
 1Kit 
KA3936
 1Kit